abstract：:To enhance efforts directed at unraveling the role and mechanisms of leukocytes in mediating injury to corneal epithelium, an isolated bovine corneal cup was developed and evaluated. Bovine peripheral leukocytes and lysates were added to the corneal epithelial surface of isolated cornea for various periods after which...

journal_title：Current eye research

authors： Elgebaly SA,Gillies C,Forouhar F,Hashem M,Baddour M,O'Rourke J,Kreutzer DL

更新日期：1985-01-01 00:00:00

abstract：:In uveal vessels of isolated arterially perfused rabbit eyes there is direct evidence for the presence of post-junctional alpha 1-receptors and indirect evidence for alpha 2-receptors. Since vasoconstriction by epinephrine could not be blocked by an alpha 1-antagonist, only 30% by an alpha 2-antagonist and almost full...

journal_title：Current eye research

authors： van Pinxteren PC,van Alphen GW

更新日期：1985-01-01 00:00:00

abstract：:Two of the major in vitro phosphorylated polypeptides of the bovine lens have been identified. Analysis by means of two-dimensional gel electrophoresis (IEF) has demonstrated that the lens phosphorylated 57,000 and 43,000 dalton polypeptides correspond in mobility to purified phosphorylated bovine lens vimentin and ch...

journal_title：Current eye research

authors： Sredy J,Roy D,Spector A

更新日期：1984-12-01 00:00:00

abstract：:High pressure liquid chromatography was used to measure alpha-tocopherol in the retinas of rats reared in a cyclic light or dark environment. These measurements were performed on extracts of whole retinas during the developmental period, 18-60 days, and on isolated ROS from adult animals. Similar alpha-tocopherol dete...

journal_title：Current eye research

authors： Hunt DF,Organisciak DT,Wang HM,Wu RL

更新日期：1984-11-01 00:00:00

abstract：:Recent reports from this laboratory indicate that dopamine (DA2) agonists can lower intraocular pressure (IOP) in rabbits, monkeys and rats. This communication suggests that the cat nictitating membrane (CNM) preparation is a useful model for localizing the site(s) and mechanism(s) of action of dopamine (DA2) agonists...

journal_title：Current eye research

authors： Potter DE,Burke JA

更新日期：1984-11-01 00:00:00

abstract：:Birefringence variations, seen as regularly spaced altering light and dark rings (bands), have been observed along the length of unfixed, freshly isolated rod outer segments (ROS) of both Rana pipiens and Xenopus laevis. In our hands, the spatial frequency of the banding pattern is from 1.3-1.6u/band in Rana pipiens a...

journal_title：Current eye research

authors： Andrews LD,Mackenzie JM Jr,Basinger SF

更新日期：1984-11-01 00:00:00

abstract：:The effects of 4% epinephrine bitartrate and 0.5% timolol on ocular and optic nerve blood flow were studied in phakic and aphakic rabbit eyes using a radioactive microsphere (85Sr) technique. Either epinephrine or timolol was given topically as eight drops in the hour immediately preceding blood flow measurements (one...

journal_title：Current eye research

authors： Jay WM,Aziz MZ,Green K

更新日期：1984-10-01 00:00:00

abstract：:A method is described for the preparation of plasma membrane enriched fractions from bovine retinal pigment epithelium (RPE) by means of differential centrifugation followed by the use of self forming gradients of Percoll. A detailed analysis of the distribution of organelle specific markers (nuclei, mitochondria, lys...

journal_title：Current eye research

authors： Ottonello S,Maraini G

更新日期：1984-09-01 00:00:00

abstract：:Arachidonic acid metabolism via the cyclooxygenase pathway and the effects of aspirin and indomethacin were studied in whole retinas of rats with streptozotocin-induced diabetes. Diabetes was induced by intravenous injection of streptozotocin (60 mg/kg) and the animals were examined 5-6 weeks later. Whole retinas of n...

journal_title：Current eye research

authors： Naveh-Floman N,Weissman C,Belkin M

更新日期：1984-09-01 00:00:00

abstract：:The 'fast PIII' rod mediated photoreceptor response to flashes of monochromatic light has been investigated in isolated retinae of the RCS rat at age 30 days. A loss of both sensitivity and maximum response amplitude were found, with a concurrent slowing of the response kinetics. These observations indicate a rod abno...

journal_title：Current eye research

authors： Low JC

更新日期：1984-09-01 00:00:00

abstract：:Serum zinc and copper levels were analyzed in 26 retinitis pigmentosa patients and compared with serum zinc levels of a control group. Serum zinc levels were documented to be decreased with a mean concentration of 0.735 +/- 0.167 micrograms/ml (p less than 0.001). Serum copper levels, on the other hand, were found to ...

journal_title：Current eye research

authors： Karcioglu ZA,Stout R,Hahn HJ

更新日期：1984-08-01 00:00:00

abstract：:Phosphorylated proteins of the chick lens were identified following incubation of lenses in a medium containing 32P and subsequent analysis by gel electrophoresis. The acidic variant of the vimentin and both subunits of fodrin were phosphorylated, as were the 95 Kd and 49 Kd proteins associated with the beaded-chain f...

journal_title：Current eye research

authors： Ireland M,Maisel H

更新日期：1984-07-01 00:00:00

abstract：:The morphology and postnatal ontogeny of the major pathway for aqueous outflow in the canine eye has been examined. Normal canine chamber angles of the Beagle at one day, one through ten weeks, three, four and six months, and one and two years of age were used. Angle tissue was prepared and examined by standard techni...

journal_title：Current eye research

authors： Samuelson DA,Gelatt KN

更新日期：1984-06-01 00:00:00

abstract：:Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale cultures and as ascites in mice. The specificity of the antibo...

journal_title：Current eye research

authors： Faure JP,Mirshahi M,Dorey C,Thillaye B,de Kozak Y,Boucheix C

更新日期：1984-06-01 00:00:00

abstract：:Water-soluble (WS) and water-insoluble (WI) crystallins were prepared from calf and bovine lenses. The WS-crystallins were analyzed by capillary isotachophoresis and by immunoelectrophoresis with polyvalent and alpha-, beta-, gamma-crystallin specific antisera. The WI-crystallins were first solubilized in formamide an...

journal_title：Current eye research

authors： Bours J

更新日期：1984-05-01 00:00:00

abstract：:It has been hypothesized that an activator of Ca-ATPase co-exists with Ca-ATPase in the mouse lens. Though the Ca-ATPase activity could be measured from mouse lens homogenate, its activity could not be determined in individual soluble and insoluble fractions. The Ca-ATPase activity, however, could be detected when an ...

journal_title：Current eye research

authors： Iwata S,Shirasawa E,Takehana M

更新日期：1984-05-01 00:00:00

abstract：:Lenses from normal Wistar rats and those from a Wistar strain with X-ray-induced cataract mutation were examined electron microscopically for morphological characterization and acid phosphatase (AcPase) localization. Ocular anlages of 11 through 18 days of gestation were included in the study. A late-separating or per...

journal_title：Current eye research

authors： Anderson JW,Gorthy WC

更新日期：1984-04-01 00:00:00

abstract：:The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatm...

journal_title：Current eye research

authors： Spierer A,Kessler E

更新日期：1984-04-01 00:00:00

abstract：:EDGF, a growth factor purified from bovine retina is able to increase the rate of wound healing of rabbit corneal epithelium in a dose dependent way. Two applications a day are enough to obtain the maximum rate. The most purified preparation of EDGF is as effective as EGF or pFGF in promoting this phenomenon and allow...

journal_title：Current eye research

authors： Petroutsos G,Courty J,Guimaraes R,Pouliquen Y,Barritault D,Plouët J,Courtois Y

更新日期：1984-04-01 00:00:00

abstract：:Vernier and grating acuity were measured in infants up to 6 months of age using a two-alternative forced-choice preferential looking technique. Vernier resolution was measured using a horizontal vernier grating. The grating was presented on half of the screen while the other half contained horizontal lines which were ...

journal_title：Current eye research

authors： Manny RE,Klein SA

更新日期：1984-03-01 00:00:00

abstract：:VIP markedly stimulates intracellular cAMP accumulation in the human retinoblastoma Y-79 cell line. cAMP increased about 5-fold above the basal level with 10(-8)M VIP and reached a maximum level (about 70-fold increase) with 2 X 10(-6)M VIP. Glucagon at 6 X 10(-8)M significantly increased cAMP accumulation with a maxi...

journal_title：Current eye research

authors： Kyritsis A,Koh SW,Chader GJ

更新日期：1984-02-01 00:00:00

abstract：:The cytoskeletal proteins of the human lens were studied by SDS-PAGE, 2-D electrophoresis and immunologically. Spectrin, vimentin and actin were identified in the superficial fiber cells of human lenses even to age 87 years. These proteins are lost from the deeper cortical and nuclear fiber cells, where a broad zone o...

journal_title：Current eye research

authors： Maisel H,Ellis M

更新日期：1984-02-01 00:00:00

abstract：:The mechanism of oxidative damage to the lens through intraocular photochemical generation of superoxide and its derivatization to other oxidants such as singlet oxygen, hydroxyl radical and hydrogen peroxide has been studied. Rat lenses when organ cultured aerobically in TC 199 containing additional amounts of ribofl...

journal_title：Current eye research

authors： Varma SD,Chand D,Sharma YR,Kuck JF Jr,Richards RD

更新日期：1984-01-01 00:00:00

abstract：:From knowledge of the optical parameters of experimental animal eyes and radiometric parameters of the light source, it is possible to provide accurate retinal irradiances (dose rates). Examples are given for the rat, cat, monkey and other animals of interest. Fluorescent lamps and incandescent lamp radiances are prov...

journal_title：Current eye research

authors： Sliney DH

更新日期：1984-01-01 00:00:00

abstract：:Focused ultrasound concentrates a large amount of ultrasonic energy into a small focal zone. We have used new techniques to develop a transducer that creates intracorneal, thermally induced lesions in rabbit eyes in vitro. Histologic study shows that these lesions are confined to the stroma and that the procedure caus...

journal_title：Current eye research

authors： Mark DB,Beuerman R

更新日期：1982-01-01 00:00:00

abstract：:In an attempt to determine the factors involved in ocular pigment variation, the eyes of pallid mice (c57BL/6J-Pa/Pa), and those of littermate black mice (C57BL/6J-+/Pa) were examined ultrastructurally, histochemically, and biochemically. As controls, the eyes of black and congenic albino mice (C57BL/6J +/C2J and -C2J...

journal_title：Current eye research

authors： Ito M,Hashimoto K,Organisciak DT

更新日期：1982-01-01 00:00:00

abstract：:Samples of aqueous humor from rabbits and humans were examined for growth effects on subconfluent monolayer fibroblast cultures from ocular and extraocular tissues. At a concentration of 20% aqueous by medium volume, both homologous and heterologous aqueous stimulated DNA synthesis in fibroblasts from both species. Th...

journal_title：Current eye research

authors： Burke J,Foster S,Herschler J

更新日期：1982-01-01 00:00:00

abstract：:Na/K ATPase activity has been determined in normal and cataractous human lenses using labeled ATP as a substrate. The enzyme is distributed throughout the lens with approximately 1/3 of the total activity in the capsule-epithelium and 1/2 of the activity in the cortex. Furthermore, the activity of Na/K ATPase decrease...

journal_title：Current eye research

authors： Kobatashi S,Roy D,Spector A

更新日期：1982-01-01 00:00:00

abstract：:Posterior subcapsular cataracts (PSC) are associated with hereditary retinal dystrophy in the Royal College of Surgeons (RCS) rat model and with human retinitis pigmentosa. The relationship of lens and retinal pathology has never been explained. Previous studies of pink-eyed RCS rats aged 2.5 to 11 months had shown an...

journal_title：Current eye research

authors： Hess HH,Newsome DA,Knapka JJ,Westney GE

更新日期：1982-01-01 00:00:00

abstract：:It has been shown that illumination of rod outer segment suspension in the presence of photosensitizers (methylene blue lambda greater than or equal to 620 nm; retinal 370 less than or equal to lambda less than or equal to 390 nm) results in chemical modification of the lipid and protein components of the photo-recept...

journal_title：Current eye research

authors： Shvedova AA,Alekseeva OM,Kuliev IYa,Muranov KO,Kozlov YuP,Kagan VE

更新日期：1982-01-01 00:00:00

abstract：:Lens epithelial cells were isolated from samples of C57BL/6 and CD-1 mice at different ages between 1 and 5 months, and assayed for the level of N-acetyl-beta-glucosaminidase and their ability to replicate in-vitro. The lens epithelial cells from C57BL/6 mice had a reduced replicative potential compared to other norma...

journal_title：Current eye research

authors： Lipman RD,Muggleton-Harris AL

更新日期：1982-01-01 00:00:00

abstract：:Cytoskeletal and membrane proteins were isolated from respectively the urea-soluble and urea-insoluble fractions of the squid lens. The main cytoskeletal polypeptide has a molecular weight (63000) and an amino acid composition similar to those of vertebrate intermediate filament proteins, including mammalian lens vime...

journal_title：Current eye research

authors： Siezen RJ

更新日期：1982-01-01 00:00:00

abstract：:Using phosphorus-31 nuclear magnetic resonance (P-31 NMR) spectroscopy the rat crystalline lens was evaluated as a potential animal model for the study of lens metabolic processes relevant to the human. The P-31 NMR spectra of intact rat lenses and rat lens PCA extracts provided comparable qualitative and quantitative...

journal_title：Current eye research

authors： Kopp SJ,Greiner JV,Glonek T

更新日期：1981-01-01 00:00:00

abstract：:We measured the relative solubility of collagen in acetic acid after pepsin digestion and tentatively identified the types of collagen present in corneas of rabbits of various ages and in corneal scar tissue, using hydroxyproline assays and polyacrylamide gel electrophoretic analyses. More than 80% of the collagen in ...

journal_title：Current eye research

authors： Cintron C,Hong BS,Kublin CL

更新日期：1981-01-01 00:00:00

abstract：:An isopter of visual field charted using the Goldmann perimeter represents a conical area of visual space whose apex is at the eye of the subject and whose base intersects the hemispherical surface of the perimeter bowl. Conventional isopter plotting conceals cartographic distortion and leads to serious underestimates...

journal_title：Current eye research

authors： Kirkham TH,Meyer E

更新日期：1981-01-01 00:00:00

abstract：:Using an animal model of retinoblastoma in inbred rats and cultured human adenovirus type 12-induced retinoblastoma-like tumor cells (RAO 188), complement-dependent cytotoxicity was determined by measuring release of 3H-uridine labelled RNA. Sera from rats in which tumors did not grow after adenovirus type 12 inoculat...

journal_title：Current eye research

authors： Nishida T,Mukai N,Solish SP

更新日期：1981-01-01 00:00:00

abstract：:Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...

journal_title：Current eye research

authors： Bours J,Vornhagen R,Herlt M,Rink H

更新日期：1981-01-01 00:00:00

abstract：:The distribution of vasoactive intestinal polypeptide (VIP)- and neurotensin (NT)-like immunoreactivities in the chicken retina was investigated by immunohistochemistry. VIP- and NT-positive cells were found throughout the chicken retina. The majority of them were located in the proximal portion of the inner nuclear l...

journal_title：Current eye research

authors： Fukuda M,Kuwayama Y,Shiosaka S,Inagaki S,Ishimoto I,Shimizu Y,Takagi H,Sakanaka M,Takatsuki K,Senba E,Tohyama M

更新日期：1981-01-01 00:00:00

abstract：:Cornea endothelial cells sensitized with rose bengal and exposed to light demonstrated a wavelength dependent alteration in physiological response. Corneas exposed to 549 millimicron swelled at rates similar to controls exposed to identical energy levels of incandescent light. Corneas exposed to 451, 500, 612, and 651...

journal_title：Current eye research

authors： Hull DS,Csukas S,Green K

更新日期：1981-01-01 00:00:00

abstract：:Reduced glutathione (0.3 mM) stimulates the activity of sodium-potassium activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione, however, has no effect on Na+K+ATPase activity in the same tissue, although it does inhibit magnesium activated ATPase...

journal_title：Current eye research

authors： Whikehart DR,Soppet DR

更新日期：1981-01-01 00:00:00